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anti dcad2  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti dcad2
    Anti Dcad2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dcad2/anti-Cadherin/pmc12839969-373-38-41
    Average 94 stars, based on 25 article reviews
    anti dcad2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor
    Article Snippet: The following antibodies were used in this investigation: anti-GFP (Sigma-Aldrich, St. Louis, MO), anti-Phospho-p44/42 Map kinase (Cell Signaling Technology, Danvers, MI), anti-DCad2, anti-Dlg 4F3, anti-Elav, anti-Delta, anti-Mmp1 antibodies 14A3D2, 5H7B11, 3B8D12, and 3A6B4 (Developmental Studies Hybridoma Bank, Univ. of Iowa), anti-Active JNK (Promega, Madison, WI), anti-P35 (Novus Biologicals, Littleton, CO), anti-Phospho-Histone H3 [pSer10] (Sigma-Aldrich, St. Louis, MO), anti-cleaved caspase-3 (Cell Signaling Techology, Danvers, MA), and anti-Perlecan (provided by S. Baumgartner).

    Article Title: The Drosophila Netrin receptor frazzled/DCC functions as an invasive tumor suppressor
    Article Snippet: The following antibodies were used in this investigation: anti-GFP (Sigma-Aldrich, St. Louis, MO), anti-Phospho-p44/42 Map kinase (Cell Signaling Technology, Danvers, MI), anti-DCad2, anti-Dlg 4F3, anti-Elav, anti-Delta, anti-Mmp1 antibodies 14A3D2, 5H7B11, 3B8D12, and 3A6B4 (Developmental Studies Hybridoma Bank, Univ. of Iowa), anti-Active JNK (Promega, Madison, WI), anti-P35 (Novus Biologicals, Littleton, CO), anti-Phospho-Histone H3 [pSer10] (Sigma-Aldrich, St. Louis, MO), anti-cleaved caspase-3 (Cell Signaling Techology, Danvers, MA), and anti-Perlecan (provided by S. Baumgartner).

    Article Title: MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos
    Article Snippet: We used the following primary antibodies: anti-Arp3 (1: 500, raised in rabbit, William Theurkauf, University of Massachusetts Medical School, Worcester, MA, USA), anti-Dia (1:1,000, raised in rabbit, Wasserman lab, University of California San Diego, La Jolla, CA, USA), anti-DCAD2 (1:1,000, RRID:AB_528120, DSHB), anti-Cortactin (1:100, raised in mouse, #610049; BD Biosciences), anti-SCAR (1:100, raised in mouse, DSHB), anti-Sqh (1:1,000, raised in rabbit, Adam C. Martin, Massachusetts Institute of Technology, Cambridge, MA, USA), anti-Rab5 and anti- Rab11 (1:500, raised in guinea pig and rabbit, respectively, Akira Nakamura, RIKEN Center for Developmental Biology, Kobe, Japan), anti-Dlg (1:50, 4F3, RRID:AB_528203, DSHB), anti-Rac1 (1:100, #AB_1553767, DSHB), and anti-RhoGEF2 (1:500, Jorg Grosshans, Phillips University of Marburg, Germany).

    Membrane:

    Article Title: MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos.
    Article Snippet: .. MIM in membrane remodeling in Drosophila embryos https://doi.org/10.1083/jcb.202502184 D ow nloaded from http://rupress.org/jcb/article-pdf/225/4/e202502184/2025533/jcb_202502184.pdf by guest on 05 February 2026 California San Diego, La Jolla, CA, USA), anti-DCAD2 (1:1,000, RRID:AB_528120, DSHB), anti-Cortactin (1:100, raised in mouse, #610049; BD Biosciences), anti-SCAR (1:100, raised in mouse, DSHB), anti-Sqh (1:1,000, raised in rabbit, Adam C. Martin, Massachusetts Institute of Technology, Cambridge, MA, USA), antiRab5 and anti- Rab11 (1:500, raised in guinea pig and rabbit, respectively, Akira Nakamura, RIKEN Center for Developmental Biology, Kobe, Japan), anti-Dlg (1:50, 4F3, RRID:AB_ 528203, DSHB), anti-Rac1 (1:100, #AB_1553767, DSHB), and anti-RhoGEF2 (1:500, Jorg Grosshans, Phillips University of Marburg, Germany). .. We also used the antibody against Drosophila MIM (1: 500, raised in rabbit) generated by Boster Bio for this study.

    Staining:

    Article Title: Differential activities of the Drosophila JAK/STAT pathway ligands Upd, Upd2 and Upd3.
    Article Snippet: Contents lists available at ScienceDirect Cellular Signalling j ourna l homepage: www.e lsev ie r.com/ locate /ce l l s ig Differential activities of theDrosophila JAK/STAT pathway ligands Upd, Upd2 andUpd3 Victoria M. Wright a,b, Katja L. Vogt b, Elizabeth Smythe b, Martin P. Zeidler a,b,⁎ a MRC Centre for Developmental and Biomedical Genetics, The University of Shef!eld, Firth Court, Shef!eld, S10 2TN, UK b Department of Biomedical Science, The University of Sheffield, Firth Court, Sheffield, S10 2TN, UK ⁎ Corresponding author at: Department of Biomedi Sheffield, Firth Court, Sheffield, S10 2TN, UK.. Tel.. : +44 1 5413.

    Bioprocessing:

    Article Title: Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue.
    Article Snippet: .. The monoclonal antibodies, anti-alpha spectrin (deposited by Branton, D. and Dubreuil, R.), anti-DCAD2 (deposited by Uemura, T.), and anti-alpha tubulin (deposited by Frankel, J. andNelsen, E. M.) were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. ..

    Immunostaining:

    Article Title: Identification of a new stem cell population that generates Drosophila flight muscles
    Article Snippet: .. For immunostaining Anti Wg (Mouse, 1:100, DSHB), Anti-Twist (Rabbit, 1:100, kindly provided by S Roth, University of Cologne), Anti-NICD (Notch intracellular C-terminal domain) (Mouse, 1:100, DSHB), Anti-Numb (Rabbit, 1:100, kindly provided by Juergen Knoblich, IMBA, Vienna), Anti-GFP (Chick, 1:500, Abcam, Cambridge, UK), Anti-CD2 (Mouse, 1:100; Serotec, Raleigh, NC, USA), TO-PRO-3-Iodide (1:1000, Invitrogen), Anti-DCAD2 (Rat, 1:200, DSHB), Anti-Beta Gal (Mouse, 1:50, DSHB), Anti-Phospho histone 3 (Rabbit, 1:100, Millipore). .. Secondary antibodies (1:500) from Invitrogen conjugated with Alexa fluor-488, 568 and 647 were used in all staining procedures.



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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with <t>N-cadherin</t> and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.
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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with <t>N-cadherin</t> and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.
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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with <t>N-cadherin</t> and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.
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    rat anti ncad  (Developmental Studies Hybridoma Bank)
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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with <t>N-cadherin</t> and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.
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    rat monoclonal anti ecad  (Developmental Studies Hybridoma Bank)
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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with <t>N-cadherin</t> and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.
    Rat Monoclonal Anti Ecad, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with N-cadherin and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.

    Journal: Nature Communications

    Article Title: Quantitative live imaging reveals PRICKLE1 controls junctional neural tube morphogenesis independent of Planar Cell Polarity

    doi: 10.1038/s41467-026-71242-0

    Figure Lengend Snippet: a Schematic showing region of interest. A: anterior, P: posterior, M: medial, L: lateral. b Location of junctional zone (JZ) from the 5ss to the 9ss in a Lifeact-EGFP embryo. c Location of JZ in fixed wildtype embryos stained with SOX2 and DAPI. d Wildtype embryo electroporated with EGFP mRNA in JZ and incubated for 48 h. JZ cells contribute to junctional NT (somite 17/18 – 27/28) and secondary NT ( > somite 27/28). e Snapshot showing individual tracks of migrating H2B-miRFP670 labelled cells. f Displacement towards the midline of cells in the medial ( n = 175 cells from 3 embryos) and lateral regions ( n = 231 cells from 3 embryos) mean, ± sem, two-sided paired t -test, p = 0.0331. g Directionality of cell trajectories in the medial ( n = 175 cells from 3 embryos) and lateral ( n = 231 cells from 3 embryos) regions, p = 8.3 × 10 −33 two-sided Kolmorogov–Sminorv test. h Cell displacement towards the midline plotted against cell starting positions across the mediolateral axis ( n = 925 tracked cells from 3 embryos) mean, ± sem. i PK1 is highly expressed in JZ compared to primary NT, mean, ± sem, (for 5ss, n = 284 cells from 3 embryos in primary NT and n = 286 cells from 3 embryos in junctional zone), p = 0.0389 (for 9ss, n = 229 cells from 3 embryos in primary NT and n = 384 cells from 3 embryos in junctional zone), p = 0.0239, two-sided paired t -test. j Immunolabelling of the primary NT and JZ with N-cadherin and PK1. k , l PK1 knockdown impairs posterior neuropore (PNP) closure, mean, ± sem, ( n = 3 embryos for both control and KD), p = 0.0932, 0.3359, 0.0174, 0.0094, 0.0017, 0.0024, for 7ss to 12ss, respectively, two-sided un-paired t -test. nt: neural tube. Asterisks indicate somite positions. Triangles indicate node position. Color scales depict pixel intensity [a.u]. Displ: displacement.

    Article Snippet: Primary antibodies used were: SOX2 (Abcam, AB97959, 1:200), PRICKLE1 (Proteintech, 22589-1-AP, 1:200), ZO-1 (Thermo Fisher Scientific, 33-9100 and 40-2200, 1;400), VANGL2 (Sigma Aldrich, MABN750, 1:50), ROCK1 (ABClonal, A11158, 1:100), p-MLC (ABClonal, AP1433, 1:100), Fibronectin (DSHB, B3/D6-s, 1:4), Fibronectin (Sigma Aldrich, F3648, 1:100), SLUG (New England Biolabs, 9585 T, 1:100), N-Cadherin (DSHB, 6B3-s, 1:100), E-Cadherin (BD Biosciences, 610181, 1:100).

    Techniques: Staining, Incubation, Knockdown, Control